Molecular cloning and characterization of a cDNA encoding extracellular signal-regulated kinase from Litopenaeus vannamei
Extracellular signal-regulated kinase (ERK) is a serine/threonine-specific kinase,which is activated by downstream signaling molecules of cellular activation,cytokine and chemokine stimulation and various other stimuli.Here we cloned an ERK gene from Litopenaeus vannamei and designated it as lverk.The lverk cDNA contained an open reading frame of 1098 bp encoding 365 amino acids.LVERK had a conserved TEY motif and serine/threonine protein kinase (S_TKc) domain,and close phylogenetic relationship to Penaeus monodon and Marsupenaeus japonicus ERK.Immunofluorescence staining analysis showed that following serum stimulation LVERK was located in cytoplasm and nucleus,but phospho-LVERK was prominently in nucleus,suggesting conserved ERK signaling module occurred in shrimp cells.Then during the white spot syndrome virus (WSSV) infection,LVERK and phospho-LVERK increased at the early stage of infection.Once silencing of lverk in vivo,the replication of WSSV was obviously inhibited.Moreover,treatment of mitogen-activated protein kinase kinase inhibitor in vitro could result in reduction of WSSV proliferation and delay of viral early gene transcription.Our results indicated a role of LVERK involved in WSSV infection.Understanding how WSSV influences ERK signaling pathway to dismantle an effective immune response may lead to insight into pathogenic progression and possible disease control.
Hong Shi Xinfu Yan Xun Xu Lingwei Ruan
Key Laboratory of Marine Biogenetic Resources,Third Institute of Oceanography,State Oceanic Administ Key Laboratory of Marine Biogenetic Resources,Third Institute of Oceanography,State Oceanic Administ
国内会议
福建省海洋学会2014年学术年会暨福建省科协第十四届学术年会分会场
福建平潭
英文
555-564
2014-09-01（万方平台首次上网日期，不代表论文的发表时间）