Molecular cloning and characterization of a threonine/serine protein kinase lvakt from Litopenaeus vannamei
The phosphatidylinositol 3-kinase (PI3K)-AKT pathway is involved in various cellular functions,including anti-apoptosis,protein synthesis,glucose metabolism and cell cycling.However,the role of the PI3K-AKT pathway in crustaceans remains unclear.In the present study,we cloned and characterized the AKT gene lvakt from Litopenaeus vannamei.The 511-residue LVAKT was highly conserved; contained a PH domain,a catalytic domain and a hydrophobic domain; and was highly expressed in the heart and gills of L.vannamei.We found,using Real-Time Quantitative PCR (Q-PCR) analysis,that lvakt was up-regulated during early white spot syndrome virus (WSSV) infection.Moreover,the PI3K-specific inhibitor,LY294002,reduced viral gene transcription,implying that the PI3K-AKT pathway might be hijacked by WSSV.Our results therefore suggest that LVAKT may play an important role in the shrimp immune response against WSSV.
AKT,Litopenaeus vannamei WSSV inhibitor
Ruan Lingwei Liu Rongdiao Shi Hong Li Fuying Xu Xun
Key Laboratory of Marine Biogenetic Resources,Third Institute of Oceanography,State Oceanic Administration,Xiamen 361005,PR China
国内会议
福建省海洋学会2014年学术年会暨福建省科协第十四届学术年会分会场
福建平潭
英文
565-577
2014-09-01（万方平台首次上网日期，不代表论文的发表时间）