Sporosarcina siberiensis sp.nov., isolated from the East Siberian Sea

摘要：

　　A Gram-stain positive,non-motile,rod-shaped bacterium,designated strain 1111S-42T,was isolated from the East Siberian Sea.The organism was found to grow at 4-30℃,pH 7.0-8.5 and in 0-8%(w/v) NaCl,with optimal growth occurring at 28 ℃,pH 7.5 and in 1%NaCl.Based on 16S rRNA gene sequence similarity studies,strain 1111S-42T was found to belong to the genus Sporosarcina and to be most closely related to Sporosarcina contaminans CCUG53915T (97.3%) and Sporosarcina soli I80T (97.2%).The main polar lipids were found to include diphosphatidylglycerol,phosphatidylglycerol and phosphatidylethanolamine.The predominant menaquinone was identified as MK-7.The major cellular fatty acids were identified as anteiso-C15:0 (34.4%),iso-C15:0 (29.8%) and anteiso-C17:0 (22.4%).The DNA G+C content of strain 1111S-42T was determined to be 39 mol%.The values of DNA-DNA relatedness between the strain 1111S-42T and related type strains of the genus Sporosarcina were less than 30%.Based on the phylogenetic analysis,along with extensive physiological and chemotaxonomic testing,we conclude that the bacterium represents a novel species of the genus Sporosarcina,for which the name Sporosarcina siberiensis sp.nov.is proposed.The type strain is strain 1111S-42T (=CGMCC 1.12516T= LMG 27494T).

关键词： Sporosarcina siberiensis sp. nov. Novel species Polyphasic taxonomy Sediment

作者: Gaiyun Zhang Huihui Ren Xiu Chen Yubian Zhang Yanliu Yang Shuang Wang Yi Jiang

作者单位: Key Laboratory of Marine Biogenetic Resources,Third Institute of Oceanography,State Oceanic Administ Yunnan Institute of Microbiology,Yunnan University,Kunming 650091,Yunnan,P.R China

会议类型: 国内会议

会议名称: 福建省海洋学会2014年学术年会暨福建省科协第十四届学术年会分会场

会议地点: 福建平潭

会议语种:英文

页码: 825-834

在线出版日期: 2014-09-01（万方平台首次上网日期，不代表论文的发表时间）