The role of Litopenaeus vannamei p38 in White Spot Syndrome Virus infection
p38 as a member of MAPK family,has been conversed from yeast to mammals.It has been implicated in numerous biological processes,including the responses to stress and inflammation.In this study,three closely related p38 MAPK homologues,lvp38a,lvp38b and lvp38c,which differ only in an internal 25-amino acid sequence,have been cloned from Litopenaeusvannamei.Three isoforms shared p38 conserved TGY motif and catalytic center,as well as had maximum identities to human p38α and Drosophila p38b.Tissue distribution revealed that lvp38a and lvp38b were ubiquitously expressed in most tissues,while lvp38c showedat relatively low levels and in a tissue-specific manner.Western blotting analysis showed that lvp38 was activated by phosphorylation during WSSV infection.Furthermore,silencing lvp38 mediated by specific dsRNA in shrimpspromoted white spot syndrome virus (WSSV) replication and viral gene transcription at the early stage.These results demonstrated that lvp38 was involved in WSSV infection and might participate in host defense at the early stage.
p38 Litopenaeus vannamei WSSV Gene silencing Host defense
Hong Shi Lingwei Ruan Xinfu Yan Defu Yao Xun Xu
Key Laboratory of Marine Biogenetic Resources,Third Institute of Oceanography,State Oceanic Administ Key Laboratory of Marine Biogenetic Resources,Third Institute of Oceanography,State Oceanic Administ
国内会议
福建省海洋学会2014年学术年会暨福建省科协第十四届学术年会分会场
福建平潭
英文
835-849
2014-09-01（万方平台首次上网日期，不代表论文的发表时间）