Rapid detection of Cronobacter sakazakii in dairy food by biofunctionalized magnetic nanoparticle based on nuclear magnetic resonance

摘要：

　　In order to ensure the safety of infant formula powder in China,a rapid and sensitive detection method for food-borne bacteria is urgently needed.We have developed a reliable immunoassay based on nuclear magnetic resonance for the specific detection of Enterobacter sakazakii in dairy samples with biofunctionalized magnetic nanoparticles.This method is able to detect Cronobacter sakazakii in milk powder and cheese samples at 1.1 to 11 MPN using the most-probable-number (MPN) assay,within an incubation period of less than 2 h.Longer incubation time (>4 h) or higher pH (>7) will decrease the sensitivity of this method.This method does not fit for the detection of bacteria at higher concentrations (>1100 MPN).This method has great potential to becomea useful tool for the rapid detection of bacterial contaminations in food,environmental and agricultural samples.

关键词： Immunoassay Magnetic nanoparticles Cronobacter sakazakii Magnetic relaxation

作者: Yu Zhao Yuting Yao Ming Xiao Yan Chen Charles C.C.Lee Li Zhang Kelvin Xi Zhang Shiping Yang Ming Gu

作者单位: Department of Life and Environment Science College,Shanghai Normal University,100 Guiling Road,Shang Department of Life and Environment Science College,Shanghai Normal University,100 Guiling Road,Shang School of Environmental and Life Sciences,University of Newcastle,Singapore 169567,Singapore Howard Hughes Medical Institute,Department of Pathology and Laboratory Medicine,David Geffen School Howard Hughes Medical Institute,Department of Biological Chemistry,David Geffen School of Medicine,U Shanghai Entry-Exit Inspection and Quarantine Bureau of the People’s Republic of China,1208,Mingshen

会议类型: 国内会议

会议名称: 第五届全国低场核磁共振技术与应用研讨会

会议地点: 上海

会议语种:英文

页码: 66-73

在线出版日期: 2013-10-11（万方平台首次上网日期，不代表论文的发表时间）