Microbial degradation mechanism and pathway of the novel herbicide pyribambenz propyl by a newly isolated Amycolatopsis sp.M3-1

摘要：

　　Propyl 4-(2-(4,6-demethoxypyrimidin-2-yloxy)benzylamino) benzoate (ZJ0273), is a novel broad-spectrum herbicidedeveloped for oilseed crop.In this study, fifteen distinct isolates capable of utilizing ZJ0273 as the sole carbon source were isolated from crop soil.One of the isolates, belonged to the family Amycolatopsis and designatedAmycolatopsis sp.M3-1, had higher ZJ0273-degarding ability and was used for the further study.At 30 ℃ and pH 7.0, degradation rate of ZJ0273 by Amycolatopsis sp.M3-1 could reach 59.3% and 68.5 % in 25 day and 60 day, respectively.In order to identify the intermediates and study biodegradation pathway of ZJ0273 by Amycolatopsis sp.M3-1, 14C labeled on different rings, i.e., ”4,6-14C-pyrimidine” ZJ0273 and ”U-14C-benzyl” ZJ0273, ultralow liquid scintillation counting and LC-MS/MS were used in the biodegrading metabolism experiments in aquatic system.

关键词： ZJ0273 biodegradation pathway degradation intermediate Amycolatopsis radioisotope labeled

作者: Shanshan Li Wenjie Zhang Jinyan Cai Yucai He Liqun Wang Zhiqiang Cai

作者单位: Lab.of Applied Microbiology and Biotechnology,Changzhou University,Changzhou 213164,China

会议类型: 国内会议

会议名称: 第五届全国微生物基因组学及合成生物学学术研讨会暨陈华葵先生100周年纪念大会

会议地点: 武汉

会议语种:英文

页码: 41-42

在线出版日期: 2014-05-16（万方平台首次上网日期，不代表论文的发表时间）