Benzimidazole Resistance of Gibberella zeae Conferred by Site-Directed Mutagenesis of Its-tubulin
Benzimidazole resistance of Gibberella zeae.(anonmorph: Fusarium graminearum) was conferred by point mutation in β2-tubulin gene (FGSG_ 06611.3, designated as β2tub), rather than β1-tubulin gene (FGSG_ 09530.3, designated as β1tub).Comparing with the β2tub, however, the β1tub of G.zeae had a much higher ho mology to the β-tubulin genes of other plant pathogenic fungus, which conferred the resistance to benzimidazole fungi cides.To determine whether the β1 tub of G.zeae has the potential ability to confer carbendazim-resistance, the site directed mutagenesis followed by gene replacement was used to change the β1tub of wild-type G.zeae strain 2021 at residue 50 (Tyr to Cys), residue 167 (Phe to Tyr), residue 198 (Glu to Lys) and residue 200 (Phe to Tyr),respectively.Comparing with 2021-derived β1tub revertant strains, all the β1tub mutant strains (Tyr50Cys, Phe167Tyr, Glu198Lys and Phe200Tyr) possessed low resistance to benzimidazole fungicides, such as carbendazim, benomyl, thiabendazole and thiophanate-methyl.There were no significandy changes among 2021, 2021-derived β1tub revertant-and β1tub mutant strains in the other biological properties.Thus, the β1tub in G.zeae was still a potential resistance gene against benzimidazole fungicides.
Gibberella zeae benzimidazole resistance β1-tubulin site-directed mutagenesis
Yu Jun-jie Chen Chang-jun Zhou Li-bang Zhang Lei-gang Zhuo-ma Ge-sang Zhou Ming-guo
Institute of Plant Protection,Jiangsu Academy of Agricultural Science College of Plant Protection,Nanjing Agriculture University
国内会议
中国植物病理学会化学防治专业委员会第七届中国植物病害化学防治学术研讨会
海口
英文
88-101
2010-11-20（万方平台首次上网日期，不代表论文的发表时间）