Fluorescence Labeling of Lycium Barbarum Polysaccharide and Their Biodegradable Stability in Biological Samples
In order to further study the biological activity of LBP3 (A sub-fractions of Lycium barbarum polysaccharide),Fluorescence labeling of LBP3 and their stability in biological samples were studied.LBP3 was labeled by fluorescein of FITC with carbon acylation reaction to obtain FITC-LBP3.The labeling efficiency was evaluated by HPGPC method and the labeling rate of FITC in FITC-LBP3 molecular was calculated as 1.3% according to the calibration curve based on the FITC standards; FT-IR analysis indicated that FITC had successfully connected to LBP3 molecules; The stability of FITC-LBP3 in vitro and in vivo was investigated using HPGPC and fluorescence spectrophotometry method with fluorescent labeled FITC-LBP3,after 24h incubation in phosphate buffer,rat blank plasma and urine at 37℃,the molecular weight and the width of molecular weight distribution were not changed,that showed FITC-LBP3 was stable in vitro.After oral administration of FITC-LBP3 to rats,FITC-LBP3 was in its original form in rat plasma and rat feces,meanwhile after an intravenous administration of FITC-LBP3 to rats,FITC-LBP3 was also excreted in its original form in rat urine,that demonstrated FITC-LBP3 metabolized in its original form.
LBP fluorescence labeling stability in vitro and vivo
TANG Huali SUN Guiju
Key Laboratory of Environmental Medicine and Engineering of Ministry of Education, and Department of Key Laboratory of Environmental Medicine and Engineering of Ministry of Education, and Department of
国内会议
广州
英文
1-10
2015-05-23(万方平台首次上网日期,不代表论文的发表时间)