Effects of RNA Interference to Selenoprotein V Gene on Cytobiological Behaviors in Human Malignant Melanoma A375 Cells
To investigate the effects of RNA interference to selenoprotein V (SELV) gene on cytobiological behaviors in human malignant melanoma A375 cells.the recombinant lentivirus vector containing SELV-shRNA was constructed and transfected into A375 cells.The cells constantly expressing the shRNA was selected after incubation with puromycin.The expressions of SELV mRNA and protein were detected by quantitative real-time reverse transcription PCR (qRT-PCR) and Western blottingrespectively.The proliferation of transfected A375 cells was assayed by MTT test.Cell cycle and apoptotic rate were analyzed by flow cytometry.The expressions of ankyrin repeat and SOCS box containing 9 (ASB9) and O-acetylglucosamine transferase (OGT),two SELV functionally related factors,were determined by qRT-PCR and ELISA,respectively.Compared with the control,significantly decreased expressions of SELV mRNA and protein (P<0.05) were found in transfected A375 cells,while the apoptotic rate was increased significantly (P<0.05).However,there was no significant difference in cell cycle and proliferation between the two groups.After the SELV gene was suppressed,the mRNA and protein expressions of ASB9 and OGT were increased significantly (P<0.05).In A375 cells,down-expression of SELV gene led to increased apoptotic rate,which was accompanied by elevated expression of ASB9 and OGT genes.
selenoprotein V malignant melanoma RNA interference
HOU Bin ZHOU JiChang GONG Chunmei XU Yuanfei ZHONG Yan SHEN Xiuhua LIU Xiaoli
Shenzhen Center for Chronic Disease Control, Shenzhen, 518020, China;School of Public Health, Shangh Shenzhen Center for Chronic Disease Control, Shenzhen, 518020, China School of Public Health, Shanghai JiaoTong University, Shanghai, 200025, China
国内会议
广州
英文
1-9
2015-05-23（万方平台首次上网日期，不代表论文的发表时间）