Molecular characterization and phylogenetic analysis of Sugarcane yellow leaf virus isolates from China
(0) Sugarcane yellow leaf virus (SCYLV) (genus Polerovirus,family Luteoviridae),the causal agent of sug arcane yellow leaf disease (YLD),was first detectedin China in 2006.To assess the distribution of SCYLV in the major sugarcane-growing Chinese provinces,leaf samples from 22 sugarcane clones (Saccharum spp.hybrid) showing YLD symptoms were collected and analyzed for infection by the virus using reverse transcription PCR (RT-PCR),quantita tive RT-PCR,and immunological assays.A complete genomic sequence (5,879 nt) of the Chinese SCYLV isolate CHN-FJl and partial genomic sequences (2,915 nt) of 13 other Chinese SCYLV isolates from this study were ampli fied,cloned,and sequenced.The genomic sequence of the CHN-FJl isolate was found to share a high identity (98.4-99.1 0-10) with those of the Brazilian (BRA) genotype isolates and a low identity (86.5-86.9 0-10) with those of the CHNl and Cuban (CUB) genotype isolates.The genetic diversity of these 14 Chinese SCYLV isolates was assessed along with that of 29 SCYLV isolates of worldwide origin reported in the GenBank database,based on the full or partial genomic sequence.Phylogenetic analysis demonstrated that all the 14 Chinese SCYLV isolates clustered into one large group with the BRA genotype and 12 0ther reported SCYLV isolates.In addition,five reported Chinese SCYLV isolates were grouped with the Peruvian (PER),CHNl and CUB genotypes.We therefore speculated that at least four SCYLV genotypes,BRA,PER,CHNI,and CUB,are associated with YLD in China.Interestingly,a 39-nt deletion was detected in the sequence of the CHN-GD3 isolate,in the middle of the ORFl region adjacent to the overlap between ORFl and ORF2.This location is known to be one of the recombination breakpoints in the Luteoviridae family.
Genomic sequences Phylogenetic relationships Sugarcane (Saccharum spp. hybrid) Sugarcane yellow leaf virus Viral genotype Yellow leaf symptom
San-Ji Gao Yi-Hua Lin Yong-Bao Pan Mona B. Damaj Qin-Nan Wang T. Erik Mirkov Ru-Kai Chen
Key Laboratory of Sugarcane Biology and Genetic Breeding,Ministry of Agriculture,Fujian Agriculture USDA-ARS,Mid-South Area,Sugarcane Research Unit,Houma,LA 70360,USA Department of Plant Pathology and Microbiology,Texas AgriLife Research,Texas A&M System,Weslaco,TX 7 Guangzhou Sugarcane Industry Research Institute,Guangzhou 510316,Guangdong,China
国内会议
海口
英文
120-129
2014-08-01（万方平台首次上网日期，不代表论文的发表时间）