Generation of a BAC based recombinant Marek”s disease virus expressing reticuloendotheliosis virus env protein as a polyvalent live vaccine
(0) Marek”s disease virus (MDV) and reticuloendotheliosis virus (REV) can cause immune suppression in chickens and lead to great economic loss in the poultry industry.To protect chickens against both pathogens,we sought to generate recombinant Marek”s disease virus type l (rMDV1) expressing the env protein of REV The complete genome of the MDV strain CVI988 was cloned as a bacterial artificial chromosome (BAC) by co-transfection with a BAC transfer vector and MDV CVI988 total DNA,and then purified by four rounds of selective passage.The infectivity of the selected BAC DNA clone was validated by MDV reconstitution in chicken embryo fibroblasts transfected with CVI988-BAC extracted from recombinant Escherichia coli.The rCVI988 had similar replication rates in vitro and pathogenicity in vivo to those of its parental strain,CVI988,whereas the rCVI988-env,which was constructed by inserting the REV env-expressing cassette into the USIO region of the CVI988 genome,replicated less than CVI988 and rCVI988.The protective efficacies ofrCVI988 and CVI988 against very virulent MDV Md5 chaUenge were similar,although the recombinant MDV-REV vaccine,rCVI988-env,has a lower protective efficacy against Md5 than the parental vaccine,it could protect a higher percentage of chickens against virulent REV challenges.Therefore,the strategy of construction of this recombinant MDV-BAC may lead to the development of recombinant vaccines against multiple viral infections.
陈瑞爱 郭凯 张正伟 王建立
国内会议
第五届中国兽药大会(中国畜牧兽医学会动物药品学分会2014年学术年会、中国畜牧兽医学会生物制药学分会暨中国微生物学会兽医微生物学专业委员会联合学术论坛)
沈阳
英文
125-136
2014-09-13（万方平台首次上网日期，不代表论文的发表时间）