Host-Pathogen Interactions of Actinobacillus Pleuropneumoniae with Porcine Alveolar Macrophage
(0) Host-pathogen interactions are of great importance in understanding the pathogenesis of infectious microorganisms.Actinobacillus pleuropneumoniae (APP)is the etiological agent of porcine pleuropneumonia,a respiratory disease which causes great economic losses worldwide.We developed in vitro models to study the host-pathogen interactions of APP using alveolar macrophage,we showed that A.pleuropneumoniae adhered and invasioned alveolar macrophage,activated of MAPK signal transduction pathway.At the same time,alveolar macrophage secreted inflammatory cytokines such as IL-8.Cell death ultimately occurs by late apoptotic,not early apoptosis.In order to identify genes that are expressed during infecting the alveolar macrophage,we undertook transcript profiling experiments with an A.pleuropneumoniae cDNA microarray using bacterial mRNAs from serotype 5b L20 strain after infected porcine alveolar macrophage or growth in BHI medium.AppChip contained 2868 probes based on the genomic sequence of App serotype Sb strain L20.Nearly all A.pleuropneumoniae transcripts could be detected on our microarrays,contained 1467 up-regulated genes and 1401 down-regulated genes,after analysised we obtained 185 genes were deemed significant differentially expressed during infecting the alveolar macrophage(p < 0.05),contained 92 gene up-regulated and 93 gene down-regulated.The up-regulated gene involved in energy metabolism,gene transcription and translation,virulence related gene such as ApxⅢ,autotransporter adhesin and so on,the down-regulated gene involved in amino acid metabolism,metabolism of cofactors and vitamins,but the ability of ABC transporters and virulence related gene such as ApxⅡ,capsular polysaccharide were decreased,So,the ApxⅢ and autotransporter adhesion may play the important role during the APP infecting alveolar macrophage.This study revealed the interactions and A.pleuropneumoniae gene expression,including virulence factors,were altered during interacting with alveolar macrophage.The differential regulation of A.pleuropneumoniae gene expression and change of alveolar macrophage after infected could foundated for the studying of pathogenesis of A. pleuropneumoniae.
Actinobacillus pleuropneumoniae Host-pathogen interactions gene expression
Lei Wang Wanhai Qin Qiuyue Ma Wenyu Han Liancheng Lei
College of Veterinary Medicine,JiLin University,Changchun,130062
国内会议
第五届中国兽药大会(中国畜牧兽医学会动物药品学分会2014年学术年会、中国畜牧兽医学会生物制药学分会暨中国微生物学会兽医微生物学专业委员会联合学术论坛)
沈阳
英文
188-207
2014-09-13（万方平台首次上网日期，不代表论文的发表时间）