Physical exercise improves functional recovery through mitigation of autophagy, apoptosis and enhancement of neurogenesis in rats after MCAO
Background It is well known that physical exercise is beneficial to functional recovery after stroke,but the mechanisms are poorly understood.Transient focal cerebral ischemia induces autophagy,apoptosis and neurogenesis in the peri-infarct region,all of which are related with the recovery of damaged brain.This study is aimed to examine the effect of physical exercise on autophagy,apoptosis and neurogenesis in the peri-infarct region in rat model of transient middle cerebral artery occlusion (MCAO).Results We found that autophagy marker microtubule-associated protein 1A light chain 3-II (LC3-II) was increased following cerebral ischemia,and physical exercise markedly reduced the expression of LC3-II in the peri-infarct region.The number of TUNEL-positive (deoxynucleotidyl transferase-mediated dUTP in situ nick-end labeling,an apoptosis marker) cells decreased,while Ki67-positive (a proliferative marker) and insulin-like growth factor-1 (IGF-1) increased more quickly in physical exercise group in comparison with that in control group after ischemia.Furthermore,LC3-punctates co-located with TUNEL-positive cells in the peri-infarct region.Conclusions The present data demonstrates that physical exercise enhances neurological function possibly by reducing autophagosomes accumulation,mitigating apoptosis as well as promoting neurogenesis in the peri-infarct region after transient cerebral infarction in rats.
Physical exercise Autophagy Apoptosis IGF-1 Neurogenesis MCAO
Liying Zhang Xiquan Hu Jing Luo Lili Li Ruxun Huang Zhong Pei
Department of Rehabilitation Medicine,the Third Affiliated Hospital,Sun Yat-sen University,Guangzhou Department of Neurology,the First Affiliated Hospital,Sun Yat-Sen University,No.58 Zhongshan Road Ⅱ,
国内会议
第四届粤港澳台物理医学与康复学学术会议暨2013年广东省医学会物理医学与康复学学术会议
广州
英文
200-209
2013-08-16（万方平台首次上网日期，不代表论文的发表时间）