Efficacy of 50 Hz electromagnetic fields on human epidermal stem cells transplantation seeded in collagen sponge scaffolds for wound healing in a murine model
Purpose: Our previous studies found that epidermal stem cells (ESC) proliferation could be promoted when exposed to 50 Hz electromagnetic fields (EMF) in vitro.In addition,we also found that collagen sponge scaffolds can serve as a delivery vehicle for ESC.In order to explore the possible efficacy of EMF for skin tissue engineering,the effects of EMF exposure on ESC transplantaion seeded in collagen sponge scaffolds for wound healing in a murine model were investigated.Materials and methods: The wound models of a full-thickness defect established with 36 7~8-week-old nude mice were randomly divided into three groups: a control group,an ESC-only group,and an ESC with EMF exposure group (frequency of 50 Hz,magnetic induction of 5 mT,60 min per day for 20 d).ESC were separated from human foreskin and cultured in vitro,and then transplanted with collagen sponge scaffolds as a delivery vehicle to wounds of the ESC-only group,and ESC with EMF exposure group was exposed to EMF after ESC transplantaion.The effects of EMF on morphological changes and the expression of β1 integrin in regenerated skins were observed.The wound healing rates and healing times were collected to evaluate the efficacy of repairment.Results: Human ESC were successfully transplanted to nude mice which fascillitated the formation of intact skin on nude mice.In contrast to the other groups,the wound healing of ESC with EMF exposure group was the fastest (P < 0.05),the structure of regenerated skins was more mature,and it contained more continuity in the number of viable cell layers and rich hair follicles structure.Conclusions: The use of 50Hz EMF as a non-invasive treatment can accelerate the wound healing of ESC transplantaion,and restore the structural integrity of regenerated skin.
Electromagnetic fields Epidermal stem cells Transplantation Wound healing Scaffold
Wen-fang Bai Hong-xiang Zhu Wei-cheng Xu Hong Huang Bo Wu Ming-sheng Zhang
Department of Rehabilitation Medicine,Guangdong general hospital,Institute of Geriatric Medicine,Gua School of Information,University of South Florida,Tampa,FL 33613,USA
国内会议
第四届粤港澳台物理医学与康复学学术会议暨2013年广东省医学会物理医学与康复学学术会议
广州
英文
220-227
2013-08-16（万方平台首次上网日期，不代表论文的发表时间）