Paeonol attenuates advanced oxidation protein product-induced oxidative stress injury in THP-1 macrophages
Ethnopharmacological relevance: Paeonol (2′-hydroxy-4′methoxyacetophenone),is thought to possess a broad range of clinical curative effects that are likely mediated by its anti-inflammatory and anti-oxidant activities.Aims of the study: To elucidate the efficacy of paeonol”s anti-inflammatory and anti-oxidant activities and the underlying mechanism of paeonol in AOPP (advanced oxidation protein product)-stimulated THP-1 macrophages.Materials and methods: After incubating the cells with AOPP plus paeonol,nitric oxide (NO) production and the levels of iNOS,RAGE (Receptor for advanced glycation end products),CD36,SR-A,SR-B1 were calculated.Moreover,THP-1 macrophages were pre-incubated with paeonol,a free radical scavenger N-acetylcysteine (NAC),NADPH oxidase inhibitor (apocynin,DPI),a specific inhibitor of nuclear factor kappa-B (NF-κB) pyrrolidine dithiocarbamate (PDTC,) prior to incubation with AOPP,then intracellular ROS (reactive oxygen species) production and quantification of tumor necrosis factor-α (TNF-α),interleukin-1β (IL-1β),interleukin-6 (IL-6),MCP-1(monocyte chemotactic protein 1) were determined.Results: Paeonol increased NO production and the mRNA level of iNOS (inducible nitric oxide synthase),whereas decreased ROS production.ROS production was also effectively attenuated by apocynin,DPI,NAC and PDTC.Furthermore,these inhibitors and paeonol could down-regulate pro-inflammatory cytokine (TNF-α,IL-1β,IL-6 and MCP-1) mRNA and protein levels.Paeonol significantly reduced RAGE and CD36 gene expressions but increased SR-A and SR-B1 gene expressions.Conclusions: These results indicate that Paeonol could decrease inflammation cytokines in THP-1 macrophages likely through a RAGE,CD36,SR-A and SR-B1-mediated signals involving NADPH oxidase dependent ROS generation.This suggests that paeonol might be using as a therapeutic agent for the diseases contributed to oxidative stress injury.
paeonol THP-1 macrophages AOPP oxidative stress inflammation
Ping Mao Shaolian Song Liqian Mo Xiaoyan Xiao Wei Xiao Waijiao Tang Xixiao Yang
Department of Pharmacy,Nanfang Hospital,Southern Medical University,No. 1838,Guangzhou Boulevard(North),Guangzhou 510515,PR China
国内会议
石家庄
英文
1-23
2014-10-24（万方平台首次上网日期，不代表论文的发表时间）