KIM-1 and NGAL as Biomarkers of Nephrotoxicity Induced by Gentamicin in Rats
Gentamicin is a member of Aminoglycosides which has represented highly effective antimicrobial agents especially in Gram-negative infections despite their toxic effects in the kidney.Rapid diagnosis is vital to preserve renal function and to slow down renal injury.Owing to the poor sensitivity and specificity of serum creatinine (SCr) and blood urea nitrogen (BUN),new biomarkers for earlier and more accurate detection are needed.The aim of our study was to determine whether kidney injury molecule-1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL) may be useful biomarkers in the assessment of Gentamycin-induced Nephrotoxicity in rats.In this study,the two biomarkers of renal toxicity was assessed via quantitative real-time PCR and immunohistochemistry in rats treated with Gentamicin for up to 7 days.Repeated administration of Gentamicin to male SD rats for 1,3,or 7 days resulted in a dose- and time-dependent increase in the expression of KIM-1 and NGAL.Changes in gene and protein expressions were fo und to correlate with the progressive histopathological alterations and preceded effects on traditional clinical parameters indicative of impaired kidney function.Both of the biomarkers are supported to be used as sensitive indicators of acute kidney injury caused by Gentamicn.
Gentamycin Nephrotoxicity KIM-1 NGAL
Chen Meng-Lu Fang Jing Tang Li Geng Yi Luo Qi-Hui Sun Feng-Jiao Chen Zheng-li Li Ming-yang Zeng Wen Gong Li Cheng An-chun Peng Xi
Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicin Sichuan Industrial Institute of Antibiotics in China National Pharmaceutical Group,Chengdu, Sichuan, Sichuan Primed Biological Technology Co., Ltd/ National Experimental Macaque Reproduce Laboratory, Y
国内会议
中国畜牧兽医学会兽医病理学分会第二十次学术研讨会暨中国病理生理学会动物病理生理专业委员会第十九次学术研讨会
银川
英文
167-178
2014-07-01（万方平台首次上网日期，不代表论文的发表时间）