Diagnostic value of aldolase A and cytokeratin19for ovine pulmonary adenocarcinoma
Ovine pulmonary adenomatosis (OPA) is a contagious and experimentally induced lung cancer of sheep,resembling human bronchioloalveolar carcinoma (BAC).A beta-retrovirus,called jaagsiekte sheep retrovirus (JSRV),is the pathogenesis of OPA which is originating from typeⅡpneumocytes and Clara cells.JSRV particles or antigens have never been detected in the tissues of naturally affected s heep.To identify the presence of tumor metastases,a histopathological diagnosis was performed on six sheep with typical clinical manifestations.Different analytical methods were included in this retrospective study,aiming to confirm the different expression of Aldolase A and cytokeratin 19 in OPA,which were significant markers of epithelial tumor cells.Results displayed,Aldolase A expressed 1.98-fold,2.0-fold and 1.45-fold in OPA by the analysis of immunohistochemistry,western blotting and real-time quantitative fluorescent PCR,respectively.Similarly,cytokeratin 19 expressed 2.39-fold,2.4-fold and 1.70-fold in OPA.Here,we reported that the two proteins were highly expressed in the epithelial cells of bronchioles,type Ⅱ pneumocytes and tumor cells of OPA,as well as expressed in the epithelial cells of bronchioles and typeⅡpneumocytes in non-neoplastic lung sections.These data suggest that Aldolase A and cytokeratin 19 could be potential markers for comprehensive studies to investigate the presence of neoplastic cells in OPA.The hypothesis that the depletion of Aldolase A and cytokeratin 19 in OPA tumor cells which belong to the sheep of different ages and development phases alters the cell viability and tumorigenesis could be further studied fo r the drug development of therapeutic target.
ovine pulmonary adenocarcinoma aldolase A cytokeratin 19 western blotting real-time quantitative fluorescent PCR immunohistochemistry
Xiao-lin Sun Yu-fei Zhang Shu-ying Liu Yue liu Kai-nian Guan
College of Veterinary Medicine, Inner Mongolia Agricultural University, Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture, Huhhot 010018, China
国内会议
中国畜牧兽医学会兽医病理学分会第二十次学术研讨会暨中国病理生理学会动物病理生理专业委员会第十九次学术研讨会
银川
英文
303-318
2014-07-01（万方平台首次上网日期，不代表论文的发表时间）