The immunosuppression of breast cancer cells mediated by TGF-β in exosomes from cancer cells
Exosomes derived from tumor cells play essential role in tumour progression,such as angiogenesis,tumor cells proliferation and immunoregulation,while hypoxia contributes to tumor progression such as angiogenesis and metastasis potentially through exosome secretion.In present study,we cultured several cancer cell lines under hypoxia (1% O2),and found that hypoxia promoted exosome release in the number of exosomes in the medium determined on CD63 immunoblotting.When the exosomes were applied to T cells,we found exosomes from hypoxia cancer cells suppressed T cells proliferation.As TGF-β,iNOS,and PGE2 are important factors mediated T cells suppression,so we used blocking antibodies against the three factors to exosomes,and found that blocking antibody against TGF-β could anneal the immunosuppressive effects of exosomes.These data demonstrated that hypoxia enhances the breast cancer cells secretion of exosome,which contains TGF-β and suppresses T cells proliferation.Our findings has significant implications to understand the underlying mechanisms of immunosuppression in tumor microenvironment,and potentially contribute to cancer therapeutic.
Hypoxia Tumor Migration Exosomes Breast cancer cells
Lei Rong Rong Li Shaoying Li Rongcheng Luo
Tranditional Chinese Medicine-Integrated Hospital,Southern Medical University,Guangzhou,510315,P.R.China
国内会议
第五届国际中医、中西医结合肿瘤学术交流大会暨第十四届全国中西医结合肿瘤学术大会
广州
英文
1-11
2014-07-01（万方平台首次上网日期，不代表论文的发表时间）