Expressionofprolyl4-hydroxylasebeta-polypeptideinnon-smallcelllungcancertreatedwithChinesemedicines
Objectives: To investigate the role of prolyl 4-hydroxylase beta polypeptide (P4HB) expressed in lung carcinoma and the intervention effect of Yiqi Chutan Formula (益气除痰方,YQCTF).Methods: Lung carcinoma model was established by subcutaneously inoculating LEWIS lung carcinoma cells in C57BL/6J mice.The differential expression of P4HB protein between YQCTF (3.0 g/kg/d,gavage,daily,21 days) group and control group was acquired by a 2 fluorescence difference gel electrophoresis (2D-DIGE),verified by Western blotting and identified by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF/TOF-MS).The expression of P4HB and P4HB mRNA in cultured A549 cells from cisplatin (DDP) 1.5 μg/mL group and 15% serum combined with DDP 1.5 μg/mL group were detected by cellular immunohistochemistry and RT-PCR,respectively.Results: The proteomics research discovered that one-third of differential proteins including P4HB were decreased in the YQCTF group (P<0.01).Clinical pathology and tissue microarray studies showed that P4HB expression in lung cancer tissue was stronger than adjacent tissues and normal lung epithelial (P<0.01).In the YQCTF and DDP combined groups,the expression of P4HB and P4HB mRNA in A549 cell were decreased significantly (P<0.01).Conclusion: YQCTF could inhibit the LEWIS lung carcinoma’s growth,decrease the expression of P4HB in LEWIS lung carcinoma and A549 cells.YQCTF might take effect through regulating P4HB in endoplasmic reticulum to inhibit the incidence and growth process of lung carcinoma.
Yiqi Chutan Formular prolyl 4-hydroxylase beta polypeptide unfolded protein response Chinese medicine
王淑美 林丽珠 周岱翰 周京旭 熊绍权
国内会议
第五届国际中医、中西医结合肿瘤学术交流大会暨第十四届全国中西医结合肿瘤学术大会
广州
英文
1-16
2014-07-01（万方平台首次上网日期，不代表论文的发表时间）