Preparation of amino-functionalized magnetite nanoclusters by ring-opening polymerization and application for targeted magnetic resonance imaging
Here we developed a ring-opening polymerization strategy for preparation of biocompatible and aminofunctionalized superparamagnetic iron oxide nanoclusters (SPIONCs).The functionalized SPIONCs could be further applied for folate-receptor (FR)-targeted magnetic resonance imaging (MRI).The citrate-stabilized SPIONCs were synthesized by solvothermal reaction using biocompatible trisodium citrate (Na3Cit) as the coordinating and stabilizing ligand and then the amino groups were covalently bonded to them through a ring-opening polymerization of epichlorohydrin and further treatment with ammonium hydroxide.The products were confirmed by XPS,FTIR,Zeta potential,and amino quantitative analysis.The amino-functionalized SPIONCs have good stability in aqueous solution and low cytotoxicity to cell lines.By in vitro MRI and quantitative element analysis,we demonstrated the FR-mediated delivery of the folic acid-conjugated SPIONCs (Fe3O4–FA) targeting FR-positive cell line such as human ovarian carcinoma cell.In conclusion,high magnetization,good stability,and effective FR-mediated uptake of the functionalized SPIONCs made them promising candidates for use as MRI contrast agents.
He Hu Jin Sun Guosheng Huang Xiangyuan Li Antao Dai Hong Yang Shiping Yang
The Education Ministry Key Lab of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials,Department of Chemistry,Shanghai Normal University,No. 100 Guilin Road,Shanghai 200234,People’s Republic of China
国内会议
杭州
英文
132-141
2014-10-01（万方平台首次上网日期，不代表论文的发表时间）