Antifungal activities and effect of Lactobacillus casei AST18 on the mycelia morphology and ultrastructure of Penicillium chrysogenum
The antifungal effects of Lactobacillus casei AST18 on Penicillium chrysogenum.were investigated.Viable bacteria were used to detect the minimum inhibitory concentration of lactic acid bacteria (102 CFU/mL) to inhibit the growth of P.chrysogenum.Results showed that L.casei AST18 inhibited the growth of P.chrysogenum.The mycelial morphology was also altered.The scanning electron microscopy images of P.chrysogenum exposed to L.casei AST18 further showed that the mycelia were collapsed and appeared transparent.Numerous clots were also observed in the fungal cytoplasm.The tip of the hypha was deformed and exhibited a moniliform or inflated shape.The transmission electron microscopy images of L.casei AST18-exposed P.chrysogenum showed the occurrence of autophagy and death of fungal cells.Changes in the morphological characteristics of P.chrysogenum were observed in the antifungal activity experiment with viable bacteria,but such changes were not evident in the cell-free culture filtrate of L.casei AST18.This bacterial strain showed high antifungal activities and could be used as a fungal inhibitor in food and medicine.
Antifungal Lactobacillus casei Ultrastructure Autophagy SEM
Hongjuan Li Fanhua Kong Jiaping Lv Shuwen Zhang Jing Lu Lu Liu Hankie Uluko Xiaoyang Pang Yanjun Sun Haixiao Xue Lili Zhao
Key Laboratory of Agro-Products Processing, Ministry of Agriculture, Institute of Agro-Products Proc State Key Laboratory of Dairy Biotechnology, Dairy Research Institute, Bright Dairy and Food Co., Lt
国内会议
第二届全国牛奶精细化管理高峰论坛暨“金宇保灵杯”奶牛养殖效益与牛奶品质安全提升学术研讨会
北京
英文
389-398
2014-07-01（万方平台首次上网日期，不代表论文的发表时间）