The free-exploratory paradigm as a model of trait anxiety in Kunming mice: test-retest reliability
The free exploratory paradigm has been proposed as a model of trait anxiety.By definition,trait anxiety does not vary from moment to moment,therefore any model used to evaluate it needs to give the same results in sequential trials.The aim of this study was to evaluate the test-retest reliability of free-exploratory paradigm in mice.The behavior of drug-naive,adult,male,Kunming mice was measured in the free-exploratory apparatus on two occasions,either one week apart (Experiment Ι) or four weeks apart (Experiment Π).The following parameters were evaluated: percentage of time spent in the novel compartment (Ntime%);percentage of novel compartment units visited (Ncross%);percentage of number of rears in the novel compartment (Nrear%);and attempts to enter the novel compartment (Attempts).Subsequently,the intraclass correlation coefficient (ICC) and the Kappa index (κ) were calculated for each of these parameters.Analysis of the parameter Nrear% in Experiment Ι showed the acceptable values of ICC (0.755) and κ (0.520),respectively,indicating good and fair reliability with a one-week inter-trial interval.While in Experiment Π,both ICC and κ of all the parameters failed to show a reasonable test/retest concordance,suggesting that with a four-week inter-trial interval,the reliability may diminish,or even disappear.However,our results still bring further support to free-exploratory paradigm as an animal model of trait anxiety.
free-exploratory paradigm trait anxiety animal model test-retest reliability Kunming mice
SUN Shi-guang SUN Rong ZHANG Hui-yun QIAO ming-qi
College of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Departmen Shandong Academy of Chinese Medicine,Jinan 250014,China College of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,China Key Laboratory of the Classical Theory of Traditional Chinese Medicine of Ministry of Education,Shan
国内会议
南京
英文
1-11
2014-06-10（万方平台首次上网日期，不代表论文的发表时间）