Integrated LC/MS Workflow of Labeled and Native N-Glycans Released from Proteins and Antibodies Using a Mixed-Mode Column and a Q Exactive Mass Spectrometer
(0) Glycans are widely distributed in biological systems in ‘free state’ and conjugated forms such as glycoproteins,glycolipids,and proteoglycans.The functions,including efficacy and safety of protein-based drugs such as recombinant proteins and monoclonal antibodies (mAbs),are often dependent on the structure and types of glycans attached to the proteins.The structures of glycans are quite diverse,complex,and heterogeneous due to post-translational modifications and physiological conditions.The structural characterization and quantitative estimation of glycans is highly essential in biotherapeutics and biopharmaceutical projects.However,it is tremendously challenging to comprehensively characterize glycan profiles and determine the structures of glycans.
Xiaoxi Zhang Srinivasa Rao Andreas Huhmer Yury Agroskin Chris Pohl Wei Jia Udayanath Aich Ilze Birznieks Julian Saba Xiaodong Liu Rosa Viner Zhiqi Hao Gurmil S. Gendeh
Thermo Fisher Scientific,Shanghai,China Thermo Fisher Scientific,Sunnyvale,CA,USA Thermo Fisher Scientific,San Jose,CA,USA Thermo Fisher Scientific,Beijing,China
国内会议
威海
英文
154-155
2014-04-19（万方平台首次上网日期，不代表论文的发表时间）