The Nano Silica-Induced Alternation of membrane-associated proteins in HaCaT cells by Proteomic Analysis

摘要：

　　Objective To investigate the effects of silica nanoparticles in expression profiles of membrane proteins in HaCaT cells.Methods We utilized a subcellular fractionation method to extract membrane fractions and analyzed the membrane proteomic via a two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption/ionization tandem time-of-flight in the membrane fractions of HaCaT cells treated with silica nanoparticles.Results Ten proteins were identified as differential expression in the membrane fractions of HaCaT cells treated with silica nanoparticles.Among these, 7 proteins were predicted as membrane proteins with at least one transmembrane domain.The most dominant function of the identified proteins was binding and the dominant protein class is cytoskeleton proteins.Conclusion Silica nanoparticles exposure can induce the differential expression of membrane-associated proteins.We anticipate that our findings can provide novel scientific clues to discover membrane biomarkers and elucidate membrane proteins-associated toxicological mechanisms of silica nanoparticles.

关键词： silica nanoparticle membrane proteins proteomics HaCaT cells

作者: Wen-Xu Hong Aibo Huang Zhuoying Zeng Xifei Yang Hua Xu Jianjun Liu

作者单位: Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of Guangdong Province, Medic Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of Guangdong Province, Medic College of Parmacy, Ji-nan University, Guangzhou, 510632, China

会议类型: 国内会议

会议名称: 中国毒理学会生化与分子毒理专业委员会学术会议暨环境内分泌干扰物的毒理学研究新技术新方法研讨会

会议地点: 深圳

会议语种:英文

页码: 125-134

在线出版日期: 2014-10-28（万方平台首次上网日期，不代表论文的发表时间）