HILIC-UPLC-MS/MS combined with hierarchical clustering analysis to rapidly analyze and evaluate nucleobases and nucleosides in Ginkgo biloba Leaves
Ginkgo biloba leaf extract has been widely used in dietary supplements and more recently in some foods and beverages.In addition to the well-known flavonol glycosides and terpene lactones, G.biloba leaves are also rich in nucleobases and nucleosides.To determine the content of nucleobases and nucleosides in G.biloba leaves at trace levels, a reliable method has been established by using hydrophilic interaction ultra-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (HILIC-UPLC-TQ-MS/MS) working in multiple reaction monitoring mode.11 nucleobases and nucleosides were simultaneously determined in 7 min.The proposed method was fully validated in terms of linearity, sensitivity, repeatability as well as recovery.Furthermore, hierarchical clustering analysis (HCA) was performed to evaluate and classify the samples according to the contents of the eleven chemical constituents.The established approach could be helpful for evaluation of the potential values as dietary supplements and the quality control of G.biloba leaves,which might also be utilized for the investigation of other medicinal herbs containing nucleobases and nucleosides.
hydrophilic interaction liquid chromatography nucleobases and nucleosides Ginkgo biloba leaf
Xin Yao Yu-Ping Tang Jin-Ao Duan Jian-an Bao Zongqi Cheng
Department of Pharmacy,First Affiliated Hospital of Soochow University,Suzhou,215006,China Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,Nanjing Uni
国内会议
中华中医药学会2014年医院药学分会学术年会暨世界中联中药专业委员会2014年国际学术会议
北京
英文
185-198
2014-10-01（万方平台首次上网日期，不代表论文的发表时间）