Sporicidal activities of a combination of orthophthalaldehyde and Didecyldimethylammonium chloride (DDAC)
The objective of this study was to evaluate the potential synergistic effect of ethanol on a combination of OPA and DDAC against the spores of Bacillus subtilis var.Niger.The quantitative carrier test for sporicidal testing of high-level disinfectants according to the guideline of China (Technical Standard for Disinfection 2002) was used as method.Considerable synergistic effect was observed after a 30 min treatment at 20℃.There was an augment in mean log reduction as the concentration of DDAC was increased ranging from 0.2 to 3 g 1-1 in combination with 6 g 1-1OPA.10 and 20% ethanol in combination with 6 g 1-1 OPA and 2 g 1-1 DDAC caused more than a 3 log reduction while either 6 g 1-1 OPA, 2 g 1-1DDAC and 20% ethanol alone or a combination of two of the three agents produced less than a 1 log reduction.Further, 40 min exposure time of combination of OPA, DDAC and 20% ethanol led to greater than a 5 log reduction of spores and no spore growth was observed following 60 and 90 min exposures.The synergistic sporicidal effect exhibited by ethanol on a combination of OPA and DDAC can be considered to enhance sporicidal activity for using in situations of sterilization, to reduce in-use concentration of OPA used alone, which may minimize its side effect.OPA may be a more satisfactory and the first choice agent to replace glutaraldehyde (GTA) as a high-level disinfectant for medical devices.
orthophthalaldehyde high-level disinfectant sporicidal activity synergistic effect
Guogang Yuan Qiuhua Wei Jinfeng Tie Changde Wang Lin Rao Wenfu Zhang
Evaluation and Research Center for Disinfection, Institute of Disease Control and Prevention, Academy of Military Medical Sciences, 20 Dongdajie Street, Fengtai District, Beijing 100071, PR China
国内会议
合肥
英文
74-81
2014-09-24（万方平台首次上网日期，不代表论文的发表时间）