Introduction of rice CYP714D1 gene into Populus inhibits expression of its homologous genes and promotes growth,biomass production and xylem fiber length in transgenic trees
The rice (Oryza sativa) OsCYP714D1 gene (also known as EU1) encodes a cytochrome P450 monooxygenase which functions as a gibberellin acid (GA) deactivating enzyme, catalyzing 16α,17-epoxidation of non-13-hydroxylated GAs.To understand whether it would also reduce the production of active GAs and depress the growth rate in transgenic trees, we constitutively expressed OsCYP714D1 in aspen hybrid clone Populus alba x P.berolinensis.Unexpectedly, ectopic expression of OsCYP714D1 in aspen positively regulates the biosynthesis of GAs, including the active GA1 and GA4, leading to promoted growth rate and biomass production in transgenic plants.Transgenic lines which showed significant expression of the introduced OsCYP714D1 gene accumulated higher of GA level and produced more numerous and longer xylem fibers than did the wild type (WT) plants.Quantitative real-time PCR indicated that transcriptions of most homologous PtCYP714 genes were suppressed in these transgenic lines.Therefore, the promoted GA and biomass production in transgenic trees constitutively expressing OsCYP714D1 is probably attributed to the down-regulation of the native PtCYP714 homologs involved in the GA biosynthesis pathway, although their precise functions are yet to be further elucidated.
Aspen Biomass Gibberellin acid OsCYP714D1 Populus Transgenic plants
Cuiting Wang Yan Bao Qiuqing Wang Hongxia Zhang
National Key Laboratory of Plant Molecular Genetics,Shanghai Institute of Plant Physiology and Ecolo Department of Cell Biology,The Lerner Research Institute,Cleveland Clinic,Cleveland,OH 44195,USA
国内会议
长沙
英文
405-420
2013-10-01（万方平台首次上网日期，不代表论文的发表时间）