Chimeric repressor of PtSND2 severely affects wood formation in transgenic Populus
NAC domain transcription factors are important regulators that activate the secondary wall biosynthesis in wood formation.In this work, we investigated the possible functions of a NAC family member PtSND2 (SECONDARY WALL-ASSOCIATED NAC DOMAIN PROTEIN2) using chimeric repressor silencing technology.RT-PCR, subcellular localization and transcriptional activation analyses indicated that PtSND2 is a wood-associated transcriptional factor with the predicted transcriptional activation activity, which could be inhibited by the repression domain SRDX (SUPERMAN REPRESSION DOMAIN X) in yeast.Wood formation was severely repressed in transgenic poplar plants overexpressing PtSND2-SRDX.Meanwhile, the secondary cell wall thickness of xylem fiber was restrained, and the contents of cellulose and lignin were obviously decreased in the stems of transgenic plants.Further studies indicated that expressions of a number of wood-associated genes were down-regulated in the stems of transgenic plants.Our results suggest that PtSND2 may play important roles during the secondary growth of stems in poplar.
poplar chimeric repressor PtSND2 secondary growth transgenic plant
H.H.Wang R.J.Tang H.Liu H.Y.Chen J.Y.Liu X.N.Jiang H.X.Zhang
National Key Laboratory of Plant Molecular Genetics,Shanghai Institute of Plant Physiology and Ecolo Department of Plant and Microbial Biology,University of California,Berkeley,CA 94720,USA College of Life Sciences,Qingdao Agricultural University,Qingdao 266109,China College of Life Sciences and Biotechnology,Beijing Forestry University,Beijing 100083,P.R.China;The
国内会议
长沙
英文
421-432
2013-10-01（万方平台首次上网日期，不代表论文的发表时间）