Yangjing Capsule Extract Promotes Proliferation of GC-1 Spg Cells
Objective: To investigate the effect of Yangjing Capsule (养精胶囊,YC) extract on proliferation ofGC-1 spg cells and the mechanism.Methods: GC-1 spg cells were treated with different concentrations of YC extract.MTT assay was performed to detect the cell viability.Flow eytometry was used to measure the cell cycle and apoptosis of GC-1 spg cells.Real-time PCR and Western blot were applied to determine the mRNA and protein expression of Oct-4 and Plzf.Gfrαl knockdown and LY294002 (PI3K inhibitor) were applied to explore the underlying mechanism.Results: After 48 h treatment of YC,the viability of GC-1 spg cells increased significantly and the ratio of apoptotic cells reduced significantly.It showed YC could evidently stimulate the proliferation of GC-1 spg cells.The increased mRNA and protein expression of Oct-4 and PLZF suggested YC extract could promote self-renewal of GC-1 spg cells.Both Gfrαl siRNAs and LY294002 treatments could hold back YC extract”s stimulation effects on mRNA and protein expression of Oct-4 and Plzf,and consequently,inhibit the proliferation of GC-1 spg cells induced by YC extract.Conclusions: YC extract could stimulate the proliferation of GC-1 spg cells.By binding to Gfrα1,YC extract is able to trigger the activation of PI3K pathway and finally lead to self-renewal of GC-1 spg cells.
YC extract GC-1 spg cells self-renewal Gfrα1 PI3K
Zhiqiang Wang Baofang Jin Yugui Cui Xindong Zhang Dalin Sun
Institute of Andrology,Nanjing University of Chinese Medicine,Nanjing 210023,China State Key Laboratory of Reproductive Medicine,Clinical Center of Reproductive Medicine,and the First
国内会议
珠海
英文
125-132
2014-11-28（万方平台首次上网日期，不代表论文的发表时间）