Hyperbaric oxygen preconditioning promotes neovascularization of transplanted skin flaps in rats
To determine whether Hyperbaric oxygen preconditioning (HBO-PC) promotes neovascularization by increasing Stromal cell derived factor-1 (SDF-1) and CXC chemokine receptor 4 (CXCR4) in transplanted skin flaps of rats.The epigastric pedicle skin flap was established in a rat model.Rats were randomly assigned to the following five groups: 1) sham-operated group (SH) ; 2) isehemia followed by reperfusion 3 days postoperatively group (IR3d) ; 3) ischemia followed by reperfusion 5 days postoperatively group (IR5d) ; 4) hyperbaric oxygen preconditioning and ischemia followed by reperfusion 3 days postoperatively group (HBO-PC3d) ; and 5) hyperbaric oxygen preconditioning and ischemia followed by reperfusion 5 days postoperatively group (HBO-PC5d).For the groups receiving HBO-PC, animals underwent 1 hour of HBO at 2.0 ATA in 100% O2 twice per day for 3 days consecutively prior to surgery.After perfusion, Laser Doppler perfusion imaging (LDPI) was performed, and skin flap tissue samples were harvested for histological evaluation and western blot analysis.Perfusion was significantly improved in the HBO-PC groups compared with the IR groups on postoperative 3 and 5.Microvessel density (MVD) was significantly increased by HBO-PC compared with IR groups postoperatively.Western blot analysis revealed that SDF-1 and CXCR4 expression in the HBO-PC groups was significantly increased compared with IR groups.HBO-PC promoted neovascularization via increasing expression levels of SDF-1 and CXCR4 in transplanted skin flaps of rats.
Hyperbaric oxygen preconditioning neovascularization skin flaps stromal cell derived factor-1 CXC chemokine receptor 4
Xuehua Liu Jing Yang Zhuo Li Lin Yang Cong Wang Chunjin Gao Fang Liang
Department of Hyperbaric Oxygen,Beijing Chaoyang Hospital,Capital Medical University,Beijing,100020,China
国内会议
苏州
英文
61-70
2014-11-01（万方平台首次上网日期，不代表论文的发表时间）