An iTRAQ based quantitative proteomic analysis to explore novel different proteins between nasopharyngeal carcinoma cell lines and their drug resistance cell lines
Purpose:To discover chemoresistant-associated proteins with the resistant model cell lines.Methods:we used the isobaric tags for relative and absolute quantitation(iTRAQ) approach combined with nano liquid chromatography-tandem mass spectrometry (NanoLC-MS/MS) analysis to identify proteins that were differentially expressed between nasopharyngeal carcinoma cell lines CNE1, CNE2 and their CDDP resistance cell lines CNE1DDP and CNE2DDP.Results:By three employing biological and technical replicates, we identified 817 nonredundant proteins,64 of which were differentially expressed in the two group cell lines(CNElvs.CNE1DDP and CNE2 vs.CNE2DDP).Through the bioinformatics:Go analysis,KEGG pathway and Protein-protein Interaction of differentially expressed proteins,some proteins such as HSPB1,CLIC1,ANXA1,TPM4, Plastin-2 ,PDIA1 and so on, might play an important role in the mechanisms of chemoresistance and clinical theray.Conclusion:The present data first time show that the chemotherapy resistant protein spectrum through these 4 cell lines,and may excavate novel potential biomarkers for early detection of chemoresistance in nasopharyngeal carcinoma.
iTRAQ nasopharyngeal carcinoma cheoresistance bioinformatics
WANG Kun TAO Ya WU Qiong LONG Lu CHEN Zhen YI Bin
Department of Clinical Laboratory, Xiangya Hospital, Central South University, Hunan, Changsha
国内会议
北京
英文
150-161
2014-06-12（万方平台首次上网日期，不代表论文的发表时间）