Localization and characterization of a putative TMH family protein CPSIT_0846 in Chlamydophila psittaci
Chlamydophila psittaci, an obligate intracellular pathogen, has been associated with psittacosis.Also, C.psittaci is a zoonotic agent.CPSIT_0846, a putative transmembrane head (TMH) family protein, was detected in the inclusion membrane during C.psittaci infection with antibodies raised with CPSIT_0846 fusion proteins by immunofluorescence assay.In the time-course monitoring, RT-PCR and Western blot revealed that it could be present as early as 2 h post infection (hpi), while it was visualized in inclusion membrane at 18 hpi in immunofluorescence assay, and remained in inclusion membrane throughout the growth cycle.CPSIT_0846 had indicated no significant orthologues with Chlamydia trachomatis and Chlamydophila pneumoniae, and in our experiment, recombinated CPSIT_0846 protein could not react with the serum of C.trachomatis and C.pneumoniae but could strongly recognize with the serum of C.psittaci, which revealed CPSIT_0846 is C.psittaci specific, so it could be used to diagnose human chlamydia disease cause by C.psittaci.Moreover, CPSIT_0846 could induce the expression of the inflammatory cytokines interleukin-1 Beta (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-oα) in THP-1 cells, which might be associated with chlamydia-induced inflammatory pathologies.
Chlamydophila psittaci CPSIT_0846 inclusion membrane protein inflammatory cytokines
Haiying Wu Liangzhuan Liu Zhiqing Li Chuanhao Jiang Xiaohua Ma Yimou Wu
Pathogenic Biology Institute, University of South China, 28 West Changsheng Rd., Hengyang
国内会议
北京
英文
413-421
2014-06-12（万方平台首次上网日期，不代表论文的发表时间）