BuShenYiQi(BSYQ) Granule Inhibits Atopic Dermatitis(AD) via a Differential Expression of Peripheral and Central Hypothalamic-Pituitary-Adrenal(HPA) Tone
Background: dysfunction of central and peripheral Hypothalamic-Pituitary-Adrenal (HPA) axis contribute to the insufficient and insensitive of endogenous glucocorticoid which is the important pathogenesis of atopic dermatitis(AD);previous study found that Bushenyiqi Chinese herbs could improve HPA axis function.Methods: The role of peripheral and central HPA axis in the pathogenesis of AD were investigated by ovalbumin(OVA) induced AD and normal BALB/c mice,meanwhile,the effect and mechanism of BuShenYiQi granule(BSYQ) on AD mice were explored.Serum corticosterone (CORT),adrenocorticotropic hormone (ACTH),corticotropin-releasing hormone (CRH) and inflammatory cytokines were determined by ELISA.In addition,the research also focused on the changes of CRF-led skin HPA axis systems.CRH/proopiomelanocortin(POMC) axis elements,corresponding functional receptors and crucial genes of glucocorticosteroidogenesis in the skin were measured by quantitative RT-PCR and Western blot.Results: We found that AD mice had a marked reduction in hypothalamic-pituitary-adrenal (HPA) tone by reduced serum CORT and ACTH,and these situations have significantly improved after BSYQ treatment.The AD mice also showed a disturbed peripheral HPA expression.Compared with normal mice,AD lesion skin were detected significantly reduced CRH mRNA and protein expression,on the contrary,POMC and cytochrome P450 side-chain cleavage enzyme (CYP11A1)were significantly increased,meanwhile,Nr3cl(GR),CRH-R2 and 11-hydroxylase type 1 (CYP 11B 1) were significantly down-regulated in the AD mice locally.Conclusions: Our results collectively suggest that AD mice displayed differential central and peripheral HPA axis tone and BSYQ significantly relieved clinical syndrome of AD mice and presented perfect anti-allergy and anti-inflammatory effects by normalizing central and peripheral HPA axis expression.
HPA axis CRH CRH-R1 CRH-R2 POMC corticosterone
Kong LW Wu JF Lin YH Chen MX Liu JQ Wang J Luo QL Ling JP Dong JC
Department of Integrative Medicine,Huashan Hospital,Fudan University,12 Middle Urumqi Road,Shanghai,200040,China;Institute of Integrated Traditional Chinese and Western Medicine,Fudan University,12 Middle Urumqi Road,Shanghai,200040,China
国内会议
第三届民族传统医学与现代医学国际学术大会暨第十三次全国中西医结合防治呼吸系统疾病学术研讨会
西宁
英文
84-91
2014-10-23（万方平台首次上网日期，不代表论文的发表时间）