Paeoniflorin attenuates allergic inflammation in asthmatic mice
Paeoniflorin (PF),one of the major active ingredients of Chinese peony,has demonstrated anti-inflammatory and immunoregulatory effects.However,it has remained unclear whether PF treatment can inhibit allergic inflammation in asthma.In this study,we evaluated the effects of PF on pulmonary function and airway inflammation in asthmatic mice.The allergic asthma models were established in BALB/c mice.The mice were sensitized and challenged with ovalbumin.Airway hyperresponsiveness was detected by direct airway resistance analysis.Lung tissues were examined for inflammatory cells infiltration.IL-5,IL-13,IL-17,and eotaxin in bronchoalveolar lavage fluid (BALF) and their mRNA expression in lung tissue were examined by ELISA and realtime PCR,respectively.The total IgE level in serum was measured by ELISA.The protein expression of p-ERK and p-JNK were detected by western blot.Our data showed that PF oral administration significantly reduced airway hyperresponsiveness to aerosolized methacholine and decreased IL-5,IL-13,IL-17 and eotaxin levels in the BALF,and decreased IgE level in the serum.Histological studies showed that PF administration markedly decreased inflammatory infiltration.Similarly,treatment with PF significantly inhibited IL-5,IL-13,IL-17 and eotaxin mRNA expression in lung tissues.The protein expression levels of 1-ERK and 1-JNK were substantially decreased after oral administration of PF.In summary,PF dis1layed anti-inflammatory effects in the OVA-induced asthmatic model by decreasing the expression of IL-5,IL-13,IL-17 and eotaxin.These effects were mediated at least partially by inhibiting the activation of MAPK pathway.
Paeoniflorin asthma IL-5 IL-13 IL-17 MAPK
Jing Sun Jinfeng Wu Changqing Xu Qingli Luo Bei Li Jingcheng Dong
Department of Integrative Medicine,Huashan Hospital,Fudan University,12 Middle Urumqi Road,Shanghai, Department of Dermatology,Huashan Hospital,Fudan University,12 Middle Urumqi Road,Shanghai,China,200 Department of respiration,Affiliated Hospital of Hangzhou Normal University,126 Wenzhou Road.,Hangzh
国内会议
第三届民族传统医学与现代医学国际学术大会暨第十三次全国中西医结合防治呼吸系统疾病学术研讨会
西宁
英文
142-143
2014-10-23（万方平台首次上网日期，不代表论文的发表时间）