Paeoniflorin attenuates oxidative stress in cigarette smoke-mediated rat model of COPD
Paeoniflorin (PF) is a monoterpene glucoside and the main component of the total glycosides” of paeony (TGP) extracted from the roots of Paeonia lactiflora which is commonly used in Traditional Chinese Medicine for centuries.More in-depth studies have reported that paeoniflorin might have an effect on the oxidative stress;however,the mechanism is still unknown.Even the relationship between the duration of effect and time remains unclear.In present study,the model of COPD was created in Sprague-Dawley(SD) rats by being exposed to tobacco smoke of 20 cigarettes for 1 hour/day,6 days/week,for either 12 weeks,24 weeks,or 36 weeks.Data from pulmonary function demonstrated rats were suffering from a continuous decline in lung function parameters which are characterized for development of COPD.Moreover,these changes come along with the histological analysis of lung tissue which presented that infiltrated inflammatory cells were significantly increased in the model group compared with NC group.Elisa result also suggested that CS induced oxidative stress in the COPD model group.Fortunately,paeoniflorin treatment can decrease the level of oxidative stress and resulted in significant ATF4 and Nrf2 expressions which are the main cause of increased level of antioxidant enzymes.All these findings together suggested that smoke inhalation can pull off the trigger of oxidative stress from the very beginning.The duration of 24-weeks treatment of paeoniflorin especially in the dosage of 40mg/kg*d worked out a better outcome at the end of the experiment partially throughout quenching ROS and up regulating antioxidant enzymes via an Nrf2-dependent mechanism.
COPD Paeoniflorin oxidative stress Nrf2
Jinpei Lin Hongying Zhang Genfa Wang Lingwen Kong Qingli Luo Yvbao Lv Jia Wang Bei Li Jingcheng Dong
Department of Integrative Medicine,Huashan Hospital,Fudan University,12 Middle Urumqi Road,Shanghai,200040,China
国内会议
第三届民族传统医学与现代医学国际学术大会暨第十三次全国中西医结合防治呼吸系统疾病学术研讨会
西宁
英文
158-166
2014-10-23（万方平台首次上网日期，不代表论文的发表时间）