High Performance Liquid chromatography-ion trap mass spectrometry characterization of steroidal saponins of Dioscoreapanthaicaand its application for accelerating the process of isolation and structural elucidation of steroidal saponins
Dioscoreapanthaicais a traditional Chinese medicinal herb which has been used to treat cardiovascular disease, gastropathy and hypertension.Steroidal saponins(SS) are the main active ingredients of this herb and have effects on myocardial ischemia, cancer, and parahormone function.Phytochemical analysis of SS, particularly isolation and structural determination, is time-consuming and laborious.Therefore, it is necessary to establish a method to speed up the process of identification, isolation and structure elucidation of SS.In this study, we developed an HPLC-IT/MS method for the identification of SS in D.panthaica.We used IT/MS to distinguish spirostanol and furostanol compounds.Spirostanol compounds were readily generated ”M+HCOO”-ina(-) ESI-MS scan, while furostanol compounds with the exception of the ions ”M+HCOO”-and”M+2HCOO”2-were indicators for the identification of this type of compound.The number, classes and the connected orders of the sugar moieties of aglucone were all studied in detail.Next, the established fragmentation features were applied for the analysis of the 65% ethanol fraction of the water extract from the dried rhizome of D.panthaica.As a result, 23 SS were identified, including eleven potential new compounds.Furthermore, two target compounds with high content were isolated by MSn analysis and their chemical structures were confirmed by 1H-, 13C-NMR.In this study, the LC-IT/MS technique was shown to be an efficient method for making the process of identification, isolation and structure determination of steroidal saponins more efficient.
Structure characterization and identification Steroidal saponins Dioscoreapanthaica LC-ESI-IT/MS
Weihao Wang Ye Zhao WenguangJing Jun zhang HuiXiao Qin Zha An Liu
Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,PR Chin Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,PR Chin
国内会议
世界中医药学会联合会方药量效研究专业委员会成立大会暨第二届国际方药量效关系与合理应用研讨会
北京
英文
317-354
2014-10-19（万方平台首次上网日期，不代表论文的发表时间）