Transport stress induces apoptosis in rat myocardial tissue via activation of the mitogen-activated protein kinase signaling pathways
The present study aimed to elucidate the mechanism of myocardial damage induced by simulated transport stress.Sprague Dawley rats were subjected to 35℃ and 60rpm (0.1 × g, rcf) on a constant temperature shaker.The blood samples was prepared for detection of epinephrine (E), norepinephrine (NE), and serum cardiac troponin T (Ctnt) ; myocardium samples was prepared for morphological examination and signaling protein quantitative.The result showed that plasma norepinephrine (NE) and epinephrine (E) concentrations increased in all stressed groups (P < 0.01).Levels of serum cardiac troponin T (Ctnt) were elevated in both the S2d (P < 0.05) and S3d groups (P <0.01).Morphological observation demonstrated obvious microstructure and ultrastructure damage after simulated transport stress.There was also a significant increase in the number of TUNEL-positive cardiomyocytes in stressed hearts.Western blot analysis found that the mitogen-activated protein kinase (MAPK) pathways were activated by strengthening phosphorylation of ASK—1 ,JNK,P38 and ERK in rat myocardial tissue after simulated transport stress (P < 0.05, P < 0.01).In addition, the ratio of pro-apoptotic Bax and anti-apoptotic Bcl—2 proleins was increased in stressed rats (P < 0.01), and the amount of cleaved-caspase3 increased in all stressed rats (P <0.01).The expression of cleaved-caspase9 protein was also elevated in S2d and S3d groups (P <0.01).Consequently simulated transport stress induced obvious myocardial damage,which may be attributed to the activation of caspase 9—mediated mitochondrial apoptotic pathway and MAPK pathways.
apoptosis myocardial damage MAPK transport stress
Wan CR Xu JQ Chen YP Yin P Han DD Xu XL He SS Liu MJ Hou XL Liu FH
CAU-BUA TCVM Teaching and Researching Team,College of Veterinary Medicine, China Agricultural Univer Beijing Key Laboratory for Dairy Cow Nutrition, Beijing University of Agriculture (BUA) ,Beijing 102
国内会议
第四届京津冀一体化畜牧兽医科技创新研讨会暨“瑞环杯”新思想、新方法、新观点论坛
石家庄
英文
90-97
2014-10-24（万方平台首次上网日期，不代表论文的发表时间）