Endoplasmic Reticulum Stress in Heat-and Shake-Induced Injury in the Rat Small Intestine
We investigated the mechanisms underlying damage to rat small intestine in heat-and shake-induced stress.Eighteen Sprague-Dawley rats were randomly divided into a control group and a 3—day stressed group treated 2 h daily for 3 days on a rotary platform at 35℃ and 60 r/ min.Hematoxylin and eosin-stained paraffin sections of the jejunum following stress revealed shedding of the villus tip epithelial cells and lamina propria exposure.Apoptosis increased at the villus tip and extended to the basement membrane.Photomicrographs revealed that the microvilli were shorter and sparser; the nuclear envelope invaginated and gaps in the karyolemma increased; and the endoplasmic reticulum (ER) swelled significantly.Gene microarray analysis assessed 93 differentially expressed genes associated with apoptosis, ER stress, and autophagy.Relevant genes were compiled from the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases.Forty-one genes were involved in the regulation of apoptosis, fifteen were related to autophagy, and eleven responded to ER stress.According to KEGG,the apoptosis pathways, MAPK signaling pathway, mTOR signaling pathway, and regulation of autophagy may have been activated.Casp3, Casp12, andLC3 increased significantly at the villus tip while mTOR decreased; phosphorylated-AKT (P—AKT) decreased.ER stress was involved and induced autophagy and apoptosis in rat intestinal damage following heat and shake stress.Bioinformatic analysis will help determine the underlying mechanisms in stress-induced damage in the small intestine.
endoplasmic reticulum heat and shake stress jejunal villi gene microarray autophagy
Peng YIN Jianqin XU Fenghua LIU Changrong WAN Shasha HE Zhaofei XIA
CAU-BUA TCVM Teaching and Researching Team, College of Veterinary Medicine, China Agricultural Unive College of Animal Science and Technology, Beijing University of Agriculture (BUA), Beijing 102206, P
国内会议
第四届京津冀一体化畜牧兽医科技创新研讨会暨“瑞环杯”新思想、新方法、新观点论坛
石家庄
英文
213-220
2014-10-24（万方平台首次上网日期，不代表论文的发表时间）