Tissue-engineered mesh for pelvic floor reconstruction fabricated from silk fibroin scaffold with adipose-derived mesenchymal stem cells
A tissue-engineered mesh fabricated with adiposederived mesenchymal stem cells (AD-MSCs) cultured on a silk fibroin scaffold is evaluated for use in female pelvic reconstruction.Thirty-five female Sprague Dawley rats were divided into four groups.Group A (n=10) were implanted with polypropylene meshes, Group B (n =10) with silk fibroin scaffolds and Group C (n =10) with tissue-engineered meshes.Group D (n=5) acted as the tissue control.The tissueengineered mesh was produced as follows.AD-MSCs were obtained from adipose tissue of rats designated to Group C.The cells were seeded onto a silk fibroin scaffold, cultured and then observed by scanning electron microscopy (SEM).Histological studies of these meshes were performed at 4 and 12 weeks after implantation and mechanical testing was carried out on all groups before implantation and at 12 weeks after implantation.AD-MSCs displayed fibroblast-like shapes and were able to differentiate into adipocytes or fibroblasts.SEM observation showed that AD-MSCs proliferated and secreted a matrix onto the silk fibroin scaffolds.After implantation of the scaffolds into rats, histological analysis revealed better organized newly formed tissue in Group C than in controls.Group C also had a similar failure force (2.67±0.15 vs 2.33±0.38 N) and a higher Young”s modulus (2.99±0.19 vs 1.68±0.20 MPa) than a normal vaginal wall, indicating the potential of this tissue-engineered approach.AD-MSCs were validated as seed cells for tissue engineering.The silk fibroin scaffold thus shows promise for application with AD-MSCs in the fabrication of tissue-engineered mesh with good biocompatibility and appropriate mechanical properties for pelvic floor reconstruction.
Adipose-derived mesenchymal stem cells Pelvic floor reconstruction Seed cells Silk fibroin scaffold Tissue engineering Rat
Qi Li Jianliu Wang Haifeng Liu Bing Xie Lihui Wei
Department of Obstetrics and Gynecology, Peking University People”s Hospital, 11 Xizhimen South Stre School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, People”s R
国内会议
北京
英文
260-269
2014-09-19（万方平台首次上网日期，不代表论文的发表时间）