Retention times and peak shapes prediction in gradient elutioned HPLC based on the plate theory

摘要：

　　The method was proposed to predict retention times and peak shapes simultaneously in gradient elution under reversed-phase high-performance liquid chromatography (RP-HPLC) based on the isocratic elutioned retention datas using the plate theory.The method can give good prediction result with different retention equations of three or four-parameter when tested in the retention prediction by 16 common sulfonamides(SAs) in 18 kinds of gradient conditions.In addition, the method of using the plate theory can well predict the change of peak shapes where caused the expansion or compression of peak under different gradient conditions.

关键词： HPLC Retention time Peak shape The plate theory Prediction

作者: WEI Xiao-bing PANG Zheng-ji FAN Guo-liang

作者单位: School of Materials Science and Engineering,Tianjin University,Tianjin 300072,China;Analysis and Tes School of Materials Science and Engineering,Tianjin University,Tianjin 300072,China

会议类型: 国内会议

会议名称: 2014第三届环渤海色谱质谱学术报告会

会议地点: 天津

会议语种:英文

页码: 617-621

在线出版日期: 2014-08-29（万方平台首次上网日期，不代表论文的发表时间）