1H NMR and HPLC Features of Chemical Composition from Species of Bupleurum
In this paper, the 1H NMR and HPLC profiles of fractionated polar extracts from samples of Bupleurum chinense were recorded and analyzed by comparing them to each other and to those of the isolated compounds.The 1H NMR spectra revealed distinct common features among samples of B.chinense collected, with the characteristic signals of their major constituents, saikosaponins, being exhibited explicitly and reproducibly.These features were further confirmed by the procedure of HPLC and online HPLC/MS analysis.And, twenty-seven saikosaponins were isolated from the extracts of the roots of B.chinense.On the basis of chemical investigation, the signals and peaks in the fingerprints were assigned to their corresponding compounds.The general features of 1H NMR spectra coupled with HPLC profiles established for authentic samples of B.chinense gave out specific data from those special compounds and can be used for original authentication.
Bupleurum chinense DC. Apiaceae saikosaponins original identification 1H NMR HPLC
Jin-Qian Yu Wen-Juan Duan Yan-Ling Geng
Shandong Analysis and Test Center,Shandong Academy of Sciences,Jinan 250014,P.R.China
国内会议
天津
英文
151-157
2014-08-29（万方平台首次上网日期，不代表论文的发表时间）