高速逆流色谱结合制备液相色谱法分离忍冬叶中黄酮苷和咖啡酰奎宁酸类成分
Nine high purity of flavonoid glycosides and caffeoylquinic acid derivatives were isolated and purified by combination of HSCCC and prep-HPLC from leaves of Lonicera japonica Thunb. The n-butanol extract was firstly isolated by HSCCC using methyl tent-butyl ether-n-butanol-acetonitrile-water (0.5％ acetic acid) (2:2:1:5,v/v), yielding five fractions F1,F2 (rhoifolin), F3 (luteoloside), F4 and FS (collected from the column after the separation). The sub-fractions F1, F4 and FS were separated by pre-HPLC successfully. Finally, chlorogenic acid (1), lonicerin (2), rutin (3), rhoifolin (4), luteoloside (5), 3, 4-O-dicaffeoylquinic acid (6), hyperoside (7), 3, S-O-dicaffeoylquinic acid (8) and 4, S-O-dicaffeoylquinic acid (9) were yielded,with the purities over 94%, respectively, as determined by HPLC.
忍冬叶 黄酮苷 咖啡酰奎宁酸类 提取分离 高速逆流色谱 制备液相色谱法 中药化学
王岱杰 耿岩玲 刘伟 刘峰 李圣波 王晓 杜金华
山东省分析测试中心,山东济南,250014 山东亚特生态技术有限公司,山东临沂,266071 山东农业大学食品与生物工程学院,山东泰安,271018
国内会议
天津
中文
374-377
2014-08-29（万方平台首次上网日期，不代表论文的发表时间）