Tongxinluo ameliorates renal structure and function by regulating miR-21-Induced epithelial-mesenchymal transition in diabetic nephropathy
Diabetic nephropathy (DN) is one of the most important diabetic microangiopathies.Epithelial to mesenchymal transition (EMT) plays an important role in DN.The physiological role of microRNA-21 (miR-21) was closely linked to EMT.However, it remained elusive whether Tongxinluo (TXL) ameliorated renal structure and function by regulating miR-21-Induced EMT in DN.This study was to determine the effect of TXL on miR-21-induced renal tubular EMT and to explore the relationship between miR-21 and TGF-β1/smads signals.Real-time RT-PCR, cell transfection and in situ hybridization (ISH) and laser confocal microscopy were used, respectively.Here, we revealed that TXL dose-dependently lowered miR-21 expression in tissue, serum and cell.Over-expression of miR-21 can enhance a-SMA expression and decrease E-cadherin expression by upregulating smad3/p-smad3 expression and downregulating smad7 expression.Interestingly, TXL also increased E-cadherin expression and decreased a-SMA expression by regulating miR-21 expression.More importantly, TXL decreased col-ⅣV, FN, GBM, GA and albumin creatinine ratio (ACR), whereas, increased creatinine clearance ratio (Ccr).The results demonstrated that TXL ameliorated renal structure and function by regulating miR-21-Induced EMT, which was one of the mechanisms to protect DN, and that miR-21 was novel therapeutic target for TXL in diabetic nephropathy.
Tongxinluo (-TXL) microRNA Epithelial to mesenchymal transition (EMT) Diabetic nephropathy
Jinyang Wang Xuan Zhao Yanbin Gao Nazhang Dawei Zou Liping Xu Jiaoyang Li Shengnan Zhou Jingjuan Yao Zhiyao Zhu
School of Traditional Chinese Medicine, capital medical university, Beijing, China
国内会议
北京
英文
1-15
2013-11-01（万方平台首次上网日期，不代表论文的发表时间）