Molecular phylogeny analysis and species identification of Dendrobium (Orchidaceae) in China
Dendrobium plants are important commercial herbs in China, widely used in traditional medicine and ornamental horticulture.In this study, sequence-related amplified polymorphism (SRAP) markers were applied in molecular phylogeny analysis and species identification of 31 Chinese Dendrobium species.Fourteen SRAP primer pairs produced 727 loci, 97% of which (706) showed polymorphism.Average polymorphism information content of the SRAP pairs was 0.987 (0.982-0.991), showing that plenty of genetic diversity exists at interspecies level of Chinese Dendrobium.The molecular phylogeny analysis (UPGMA) grouped the 31Dendrobiumspecies into six clusters.We obtained 18 species-specific markers, which can be used to identify 10 of the 31 species.Our results indicate the SRAP marker system is informative and would facilitate further application in germplasm appraisal, evolution, and genetic diversity studies in the genus Dendrobium.
Dendrobium sequence-related amplified polymorphism (SRAP) phylogeny genetic diversity
Shang-Guo Feng Jiang-Jie Lu Ling Gao Jun-Jun Liu Hui-Zhong Wang
Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants,Hangzhou Normal University, Hangzhou 310018, China
国内会议
深圳
英文
132-140
2013-08-09（万方平台首次上网日期，不代表论文的发表时间）