DNA methylation-sensitive amplified polymorphism in Phyllostachys bambusoides during flowering and rejuvenation
Methylation-sensitive amplification polymorphism was used to detect the DNA methylation status of Phyllostachys bambusoides Sieb.et Zucc during flowering and asexual rejuvenation, and analysis of methylation before and after flowering was used to reveal epigenetic changes during flowering and rejuvenation of the bamboo.Methylation level in P.bambusoides blades decreased during the flowering stage and then increased gradually after flowering when new bamboo stands were produced via asexual rejuvenation.Compared with non-flowering bamboo stands, 29.09% of methylation sites were mutated during P.bambusoides flowering, with 17.88% of sites completely demethylated in flowering plants, which was much higher than that of the methylation sites (3.61%).The number and ratio ofpolymorphic sites where changes in methylation status occurred in rejuvenated and non-flowering bamboo stands, especially demethylated sites, were lower than those in flowering bamboo stands.Methylation in floral organs was lower than that in blades in flowering P.bambusoides,and 28.58% of sites demonstrated changes in methylation status, mainly in the form of demethylation.
Phyllostachys bambusoides Sieb.et Zucc flowering rejuvenation DNA methylation MSAP
Sun Hui-Min Gu Xiao-Ping Yuan Jin-Ling Yue Jin-Jun
Research Institute of Subtropical Forestry,Chinese Academy of Forestry,Fuyang 311400,China
国内会议
安徽霍山
英文
29-39
2013-05-01（万方平台首次上网日期，不代表论文的发表时间）