Long non-coding RNAs are expressed in pancreatic ductal adenocarcinoma and IncRNA BC008363 is an independent prognostic factor in PDAC
Background:Long non-coding RNAs are a novel class of mRNA-like transcripts with no protein coding capacity, but with a variety of functions including roles in epigenetics and gene regulation.In recent reports,the aberrant expression of lncRNAs has been associated with human cancers, suggesting a critical role in tumorigenesis.Methods:In the present study, we analysis mRNAs and lncRNAs expression in pancreatic ductal adenocarcinoma (PDAC) by microarray platform, and analysis some lncRNAs expression from 30 patients who underwent pancreatectomy for pancreatic ductal adenocarcinoma by Real time PCR.We also investigated the relationship between the expression of BC008363 and the prognosis of PDAC patients by Kaplan -Meier analyses.Results:According to the data of microarrays, 1881 lncRNAs were upregulated and 3369 IncRNAs were downregulated (fold change ≥4.0 or ≤0.4, P <0.01) in the PDAC group when compared with control group.We found that the expression level of lncRNA BC008363 was significantly lower (23-fold ) in PDAC tissues when compared with corresponding nontumor pancreatic tissues, and patients with high levels of lncRNA BC008363 expression had significantly better survival rates than those with low levels of IncRNA BC008363 expression.Conclusions:These data indicate that there are different lncRNAs expressed in the pancreatic cancer biology and lncRNA BC008363 may be a novel marker for the prognosis of pancreatic cancer.
Jiao Li Yongwei Sun
Department of General Surgery,Shanghai Jiao-Tong University School of Medicine,Renji Hospital,Shanghai,China
国内会议
2014年肝胆胰外科张家港学术论坛暨第26届全国肝胆胰外科学术经验交流会
江苏张家港
英文
303-314
2014-05-30（万方平台首次上网日期，不代表论文的发表时间）