Preparation,characterization and mechanism study of goblet cell-targetingmulti-force crosslinked nanoparticles and the influence of mucus
Multi-force crosslinked trimethyl chitosan nanoparticles (M NPs) with the combination of multi ion electrostatic attraction, macromolecule entanglement and hydrophobic interaction and their goblet cell-targeting nanoparticles (M-CSK NPs) were prepared and characterized.Compared with single ion crosslinked nanoparticles (S NPs), in combined with the stability and targeting effects, these nanoparticles led to the enhanced insulin uptake and transport across the Caco-2/HT29-MTX co-cultured cells via clathrin, caveloae-mediated endocytosis and macropincytosis.Besides, both multi-force nanoparticles were able to transiently open the epithelial tight junctions among cell monolayers, which involving JNK-dependent pathway.The mucus was found to facilitate the drug transportation of both multi-force NPs.Finally, the in vivo studies in diabetic rats demonstrated that M-CSK NPs produced a stronger and more sustained hypoglycemic effect than those of S-CSK NPs and M NPs.
Jian Zhang Yun Jin Yuan Huang
Key Laboratory of Drug Targeting and Drug Delivery System, Ministry of Education, West China School of Pharmacy, Sichuan University.No.17, Block 3, Southern Renmin Road, Chengdu 610041, P.R.China
国内会议
2013年中国药物制剂大会——中国药学会药剂专业委员会2013年学术年会暨国际控释协会中国分会2013年学术年会
武汉
英文
299-302
2013-10-26（万方平台首次上网日期，不代表论文的发表时间）