Effects of Di-n-butyl Phthalate and Diethyl Phthalate on Acetylcholinesterase Activity and Neuro-related Gene Expression in Embryonic Zebrafish
In the present study, zebrafish embryos were used to assess the neurotoxicity of di-n-butyl phthalate (DBP), diethyl phthalate (DEP) and their mixture.Four-hour post-fertilization (hpf) zebrafish embryos were exposed to various concentrations of DBP, DEP and their mixture (DBP-DEP) until 96 hpf.The transcriptions levels of selected neuron-related genes reported as neurotoxicity biomarkers were analyzed.The results showed that transcripts of growth associated protein 43 (gap43), embryonic lethal abnormal vision-like 3 (elavl3), glial fibrillary acidic protein (gfap), myelin basic protein (mbp), al-tubulin and neurogeninl (ngnl) were significantly up-regulated after DBP, DEP and DBP-DEP mixture exposure.In addition, acetylcholinesterase (AChE) activity was significantly inhibited in the embryos.These results indicate that DBP and DEP have the potential neurotoxicity in zebrafish embryos.
Di-n-butyl phthalate Diethyl phthalate Developmental neurotoxicity Nervous system Zebrafish
Hai Xu Xiaoling Shao Zhen Zhang Yanmin Zou Xiangyang Wu Liuqing Yang
School of the Environment,Jiangsu University,Xuefu Rd.301,Zhenjiang 212013,Jiangsu,China School of Chemistry and Chemical Engineering,Jiangsu University,Xuefu Rd.301,Zhenjiang 212013,Jiangs
国内会议
哈尔滨
英文
533-539
2013-12-01（万方平台首次上网日期，不代表论文的发表时间）