Methylome Analysis between Divergent Tenderness of Beef in Angus Cattle
Tenderness constitutes one of the main characteristics in beef quality.In this study, a methylome analysis was performed in closed Wye Angus steers with genetically homogenous phenotypic backgrounds, to further understand the regulation of gene expression and the relationship between DNA methylation and beef tenderness.Through the use of the Methyl CpG Binding Domain protein-enriched genome sequencing (MBD) method, differentially methylated regions (DMRs) were identified and annotated, respectively.Among 1,576 identified DMRs, 983 were downregulated and 593 were upregulated in the ”tender” group, demonstrating a global decrease in DNA methylation.Finally, we found some epigenetically-regulated genes and important pathways related to beef tenderness, including calcium signaling, heparin sulfate biosynthesis, Gamma-Amino Butyric Acid (GABA) receptor signaling, mitochondrial L-carnitine shuttle and lipopolysaccharide/interleukin 1 (LPS/IL-1).The results from the research provide new insights to the regulation of genes that affect beef tenderness though DNA methylation.
DNA methylation Beef tenderness Cattle Meat quality
José A.Carrilio Chunping Zhao Jiuzhou Song
Department of Animal and Avian Sciences, University of Maryland, College Park, MD 20742, USA College of Animal and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R.China
国内会议
第二届中国肉牛选育改良与产业发展国际研讨会暨中国畜牧兽医学会养牛分会八届二次学术研讨会
陕西杨凌
英文
42-46
2013-10-22（万方平台首次上网日期，不代表论文的发表时间）