Comparisons Of Adipogenesis And Mitogenesis For Subcutaneous Fat Derived Stromal Vascular Cells Between Wagyu and Angus Cattle

摘要：

　　Japanese Wagyu cattle are well known for their extremely high marbling, with poorly defined mechanisms.The objective is to evaluate breed differences in the structure of subcutaneous (SQ) fat tissue, adipogenesis and mitogenesis of stromal vascular (SV) cells between Wagyu and Angus cattle.Histologic analysis of fat tissue indicated Wagyu had larger adipocytes and more abundant small cells compared to Angus cattle.At the tissue level, the protein expression of PPARγ in Wagyu was much lower compared to Angus (P < 0.001).Similarly, the lower mRNA expression of PPARγ was found in Wagyu SV cells (P < 0.05).As assessed by oil red-O staining, Wagyu SV cells possessed a notable trend of lower adipogenic ability (P =0.094).Interestingly, significant higher mitogenic ability was discovered in Wagyu SV cells (P =0.028), which was associated with the elevated phosphorylation of extracellular signal-regulated kinase (Erk)-1/2 (P =0.013).Moreover, exogenous fibroblast growth factor (FGF)-2 enhanced mitogenesis and Erk1/2 activity of SV cells to a greater degree in Angus compared to Wagyu (Angus: 176.42 ± 29.17% for mitogenesis and 210.00 ± 100.45% for Erk1/2 phosphorylation, P < 0.001 and P =0.003; Wagyu: 71.86 ± 31.11% for mitogenesis and 42.64 ± 33.51% for Erk1/2 phosphorylation, P =0.065 and P =0.033).Comparisons of mRNA expressions for several autocrine growth factors in Wagyu and Angus cattle showed the expression of transforming growth factor (TGF)-β3 (P =0.008) and bone morphogenetic protein (BMP)-2 (P =0.043) in Wagyu SV cells was lower than that of Angus cattle.Because both TGFβ3 and BMP2 are transforming growth factors known to inhibit proliferation, their lower expression in Wagyu might provide clues for breed differences on proliferation of SV cells in these two cattle breeds.

关键词： adipocyte adipogenesis differentiation Erk1/2 Japanese Black proliferation TGF-β3 Wagyu

作者: Min Du Shengjuan Wei Xing Fu Xingwei Liang Zhihua Jiang Steven M.Parish Michael V.Dodson Linsen Zan

作者单位: Department of Animal Sciences, Washington State University, Pullman, WA 99164, USA Department of Animal Sciences, Washington State University, Pullman, WA 99164, USA;College of Animal Veterinary Teaching Hospital, Washington State University, Pullman, WA 99164, USA College of Animal Science and Technology, Northwest A&F University,Yangling, Shaanxi, 712100, P R Ch

会议类型: 国内会议

会议名称: 第二届中国肉牛选育改良与产业发展国际研讨会暨中国畜牧兽医学会养牛分会八届二次学术研讨会

会议地点: 陕西杨凌

会议语种:英文

页码: 60-60

在线出版日期: 2013-10-22（万方平台首次上网日期，不代表论文的发表时间）